Suzhou Jiwan Gene Biotechnology Co., Ltd.

Luciferase can catalyze substrate to produce autofluorescence through enzymatic reactions, and can detect gene expression with extreme sensitivity and efficiency. In biotechnology and molecular biology, luciferase is commonly used as a reporter gene to detect gene expression or track biological processes. By delivering the luciferase gene into cells through AAV vectors, luciferase can be expressed in cells, thereby utilizing its fluorescence properties to detect gene expression or track biological processes.

The binding of spCas9 nuclease to gRNA induces double strand breaks (DSBs) in the target sequence, which are then repaired through non homologous end joining (NHEJ) or homology directed repair (HDR) pathways to achieve gene editing. rAAV is an important vector for gene delivery, but its packaging capacity is small (~4.7kb), making it difficult to express spCas9 using rAAV vectors. Our company has developed large gene AAV (lgAAV) vectors that can package the complete expression frame of spCas9 nuclease, achieving efficient expression of spCas9.

Fluorescent proteins are a type of protein that can exhibit bright fluorescence at specific wavelengths. One of the main characteristics of fluorescent proteins is that they can be directly observed in cells or in vivo. Furthermore, fluorescent proteins have characteristics such as high temperature resistance and electronic chemical stability. Our company provides a variety of fluorescent proteins, such as green fluorescent protein, red fluorescent protein, and near-infrared fluorescent protein, etc.